Computational design of a specific heavy chain/κ light chain interface for expressing fully IgG bispecific antibodies
نویسندگان
چکیده
منابع مشابه
IgG light chain-independent secretion of heavy chain dimers: consequence for therapeutic antibody production and design
Rodent monoclonal antibodies with specificity towards important biological targets are developed for therapeutic use by a process of humanisation. This process involves the creation of molecules, which retain the specificity of the rodent antibody but contain predominantly human coding sequence. Here, we show that some humanised heavy chains (HCs) can fold, form dimers and be secreted even in t...
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Bispecific antibodies: design, therapy, perspectives
Antibodies (Abs) containing two different antigen-binding sites in one molecule are called bispecific. Bispecific Abs (BsAbs) were first described in the 1960s, the first monoclonal BsAbs were generated in the 1980s by hybridoma technology, and the first article describing the therapeutic use of BsAbs was published in 1992, but the number of papers devoted to BsAbs has increased significantly i...
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Bispecific antibodies enable unique therapeutic approaches but it remains a challenge to produce them at the industrial scale, and the modifications introduced to achieve bispecificity often have an impact on stability and risk of immunogenicity. Here we describe a fully human bispecific IgG devoid of any modification, which can be produced at the industrial scale, using a platform process. Thi...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2017
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.3240